From 2010 British MotoGP will move from Donington Park to a new home at Silverstone.
The news follows the Leicestershire circuit's recent announcement that it was to stage the Formula One British Grand Prix, previously at held at Silverstone.
Simon Gillett, Donington Park chief executive, said he was disappointed at the loss and added: "Since 1987 we have continually invested in MotoGP, emotionally as well as financially.
"We would like to wish Silverstone the best of luck with the required construction and all of the work involved in staging a top flight motorcycle race and ask them to take care of an event that we have invested heavily in since we took it over with just 18,000 people attending each race."
In a statement issued by Donington Park it said: "... we are annoyed that Dorna did not give the circuit the courtesy to renew the agreement, despite reassurances to the contrary."
Over 70,000 spectators now regularly attend the event and this year's British GP will be the 23rd consecutive premier motorcycle race at Donington Park.
It is now expected this year's race could bring one of the highest attendances to date.
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